When you render a composition containing separated footage, set the Field Rendering option to the same field order as your video equipment. If you field-render with the incorrect settings, the final movie may appear too soft, jerky, or distorted. A simple test can determine the order in which your video equipment requires fields.
The test takes about 15 to 20 minutes and involves creating two movie versions of the same composition (one rendered with Upper Field First and one with Lower Field First), and then playing the movies to see which choice looks right.