In the Timeline panel, you can split a layer
at any time, creating two independent layers. This is a time-saving
alternative to duplicating and trimming the layer—something you
might do when you want to change the stacking-order position of
the layer in the middle of the composition.
Note: To make new split
layers appear above the original layer in the Timeline panel, select
Create Split Layers Above Original Layer (Edit > Preferences >
General (Windows) or After Effects > Preferences >
General (Mac OS)). Deselect this option to make the layers
appear below the original layer.